Crystallization (Comment) | Organism |
---|---|
native enzyme or enzyme complexed with L-proline, tRNAPro isoacceptors, ATP-Mn2+, prolyl-adenylate intermediate, or prolyl-adenylate analogue Pro-AMS, preparation by soaking of native enzyme crystals in ligand solutions, X-ray diffraction structure determination at 2.85-3.0 A resolution | Thermus thermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | - |
Thermus thermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-proline + tRNAPro | Thermus thermophilus | - |
AMP + diphosphate + L-prolyl-tRNAPro | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + L-proline + tRNAPro = AMP + diphosphate + L-prolyl-tRNAPro | induced fit substrate recognition, reaction mechanism, proline binding site structure | Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-proline + tRNAPro | - |
Thermus thermophilus | AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
ATP + L-proline + tRNAPro | binding of L-proline and ATP causes conformational changes in the proline binding loop and motif 2 loop, formation of an activated prolyl-adenylate reaction intermediate, required for the final conformational ordering of a ten residue peptide, the ordered loop, close to the active site | Thermus thermophilus | AMP + diphosphate + L-prolyl-tRNAPro | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Global RNA synthesis factor | - |
Thermus thermophilus |
Pro-tRNA synthetase | - |
Thermus thermophilus |
Proline translase | - |
Thermus thermophilus |
Proline--tRNA ligase | - |
Thermus thermophilus |
Prolyl RNA synthetase | - |
Thermus thermophilus |
Prolyl-transfer ribonucleate synthetase | - |
Thermus thermophilus |
Prolyl-transfer ribonucleic acid synthetase | - |
Thermus thermophilus |
Prolyl-transfer RNA synthetase | - |
Thermus thermophilus |
Prolyl-tRNA synthetase | - |
Thermus thermophilus |
ProRS | - |
Thermus thermophilus |
ProRSTT | - |
Thermus thermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Thermus thermophilus |